ARG57422
anti-ALP / Alkaline Phosphatase antibody
anti-ALP / Alkaline Phosphatase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication12
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ALP / Alkaline Phosphatase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ALP / Alkaline Phosphatase |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human ALP / Alkaline Phosphatase. |
| Conjugation | Un-conjugated |
| Alternate Names | TNSALP; HOPS; EC 3.1.3.1; Alkaline phosphatase, tissue-nonspecific isozyme; TNAP; Alkaline phosphatase liver/bone/kidney isozyme; APTNAP; AP-TNAP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HepG2 |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ALPL |
| Gene Full Name | alkaline phosphatase, liver/bone/kidney |
| Background | There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] |
| Function | This isozyme may play a role in skeletal mineralization. [UniProt] |
| Calculated MW | 57 kDa |
| PTM | N-glycosylated. |
Images (4) Click the Picture to Zoom In
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ARG57422 anti-ALP / Alkaline Phosphatase antibody IHC-P image
Immunohistochemistry: Mouse bone stained with ARG57422 anti-ALP / Alkaline Phosphatase antibody.
From Jingyuan Wen et al. Biomed Pharmacother., (2023) doi: 10.1016/j.biopha.2022.114122, Fig. 5C.
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ARG57422 anti-ALP / Alkaline Phosphatase antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57422 anti-ALP / Alkaline Phosphatase antibody.
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ARG57422 anti-ALP / Alkaline Phosphatase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer stained with ARG57422 anti-ALP / Alkaline Phosphatase antibody at 1:100 dilution.
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ARG57422 anti-ALP / Alkaline Phosphatase antibody WB image
Western blot: HepG2 cell lysate stained with ARG57422 anti-ALP / Alkaline Phosphatase antibody.
Specific References
β-catenin inhibition disrupts the homeostasis of osteogenic/adipogenic differentiation leading to the development of glucocorticoid-induced osteonecrosis of femoral head
IHC-P / Mouse
Ambient PM2.5 Exposure and Bone Homeostasis: Analysis of UK Biobank Data and Experimental Studies in Mice and in Vitro
IHC-P, WB / Mouse
Disruption of Col9a2 expression leads to defects in osteochondral homeostasis and osteoarthritis-like phenotype in mice
IHC-P / Mouse
Glycyrrhizic acid alters the hyperoxidative stress-induced differentiation commitment of MSCs by activating the Wnt/β-catenin pathway to prevent SONFH
WB / Human
Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects
IHC-P / Mouse
N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-κB Signaling
IHC-P / Mouse
Fractions of Shen-Sui-Tong-Zhi Formula Enhance Osteogenesis Via Activation of β-Catenin Signaling in Growth Plate Chondrocytes
IHC-P / Mouse
Exploring the mechanism of Bushenhuoxue formula acting on postmenopausal osteoporosis via network pharmacology and experimental validation.
IHC-P / Mouse
Platelet-rich plasma promotes bone formation, restrains adipogenesis and accelerates vascularization to relieve steroids-induced osteonecrosis of the femoral head.
IHC-P / Rat
Treated dentin matrix‐based scaffolds carrying TGF-β1/BMP4 for functional bio-root regeneration.
WB / Rat
Bushenhuoxue formula promotes osteogenic differentiation of growth plate chondrocytes through β-catenin-dependent manner during osteoporosis.
IHC-P / Mouse
Genome-wide microRNA screening reveals miR-582-5p as a mesenchymal stem cell-specific microRNA in subchondral bone of the human knee joint.
WB / Human WB / Mouse
