ARG45174
anti-ALKBH1 antibody
anti-ALKBH1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ALKBH1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ALKBH1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminal region of human ALKBH1. |
| Conjugation | Un-conjugated |
| Alternate Names | ALKBH1; alkB homolog 1, histone H2A dioxygenase; DNA lyase ABH1; EC 4.2.99.18; ABH; Alkylated DNA repair protein alkB homolog 1; DNA oxidative demethylase ALKBH1; alkB; hABH; ABH1; Alpha-ketoglutarate-dependent dioxygenase ABH1; ALKBH; EC 1.14.11.33 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 43 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ALKBH1 |
| Gene Full Name | alkB homolog 1, histone H2A dioxygenase |
| Background | This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq, Jul 2008] |
| Function | Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron. [UniProt] |
| Cellular Localization | Nucleus; Mitochondrion. [UniProt] |
| Calculated MW | 44 kDa |
Images (4) Click the Picture to Zoom In
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ARG45174 anti-ALKBH1 antibody IHC-P image
Immunohistochemistry: Human intestinal cancer stained with ARG45174 anti-ALKBH1 antibody at 1 μg/ml dilution.
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ARG45174 anti-ALKBH1 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45174 anti-ALKBH1 antibody at 2 ug/ml dilution.
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ARG45174 anti-ALKBH1 antibody WB image
Western blot: Jurkat and K562 stained with ARG45174 anti-ALKBH1 antibody at 0.25 μg/ml dilution.
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ARG45174 anti-ALKBH1 antibody FACS image
Flow Cytometry: A431 stained with ARG45174 anti-ALKBH1 antibody at 1 µg/10^6 cells dilution.
