ARG54808
anti-ALG14 antibody
anti-ALG14 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ALG14 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ALG14 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 67-93 (Center) of Human ALG14. |
| Conjugation | Un-conjugated |
| Alternate Names | UDP-N-acetylglucosamine transferase subunit ALG14 homolog; CMS15 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | NCI-H460 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96F25 Human UDP-N-acetylglucosamine transferase subunit ALG14 homolog |
|---|---|
| Gene Symbol | ALG14 |
| Gene Full Name | ALG14, UDP-N-acetylglucosaminyltransferase subunit |
| Background | This gene is a member of the glycosyltransferase 1 family. The encoded protein and ALG13 are thought to be subunits of UDP-GlcNAc transferase, which catalyzes the first two committed steps in endoplasmic reticulum N-linked glycosylation. Mutations in this gene have been linked to congenital myasthenic syndrome (CMSWTA). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015] |
| Function | May be involved in protein N-glycosylation. May play a role in the second step of the dolichol-linked oligosaccharide pathway. May anchor the catalytic subunit ALG13 to the ER. [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein |
| Research Area | Controls and Markers antibody |
| Calculated MW | 24 kDa |
Images (3) Click the Picture to Zoom In
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ARG54808 anti-ALG14 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma stained with ARG54808 anti-ALG14 antibody.
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ARG54808 anti-ALG14 antibody WB image
Western blot: 35 µg of NCI-H460 cell lysate stained with ARG54808 anti-ALG14 antibody.
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ARG54808 anti-ALG14 antibody FACS image
Flow Cytometry: NCI-H460 cells stained with ARG54808 anti-ALG14 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
