ARG59775
anti-ALDH4A1 antibody
anti-ALDH4A1 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ALDH4A1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ALDH4A1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 354-563 of Human ALDH4A1 (NP_003739.2). |
| Conjugation | Un-conjugated |
| Alternate Names | P5CD; ALDH4; P5CDh; Aldehyde dehydrogenase family 4 member A1; L-glutamate gamma-semialdehyde dehydrogenase; Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial; P5C dehydrogenase; EC 1.2.1.88 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse skeletal muscle and BT474 | ||||||
| Observed Size | 65 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30038 Human Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Swiss-port # Q8CHT0 Mouse Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial |
|---|---|
| Gene Symbol | ALDH4A1 |
| Gene Full Name | aldehyde dehydrogenase 4 family, member A1 |
| Background | This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009] |
| Function | Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes. [UniProt] |
| Cellular Localization | Mitochondrion matrix. [UniProt] |
| Calculated MW | 62 kDa |
Images (2) Click the Picture to Zoom In
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ARG59775 anti-ALDH4A1 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG59775 anti-ALDH4A1 antibody.
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ARG59775 anti-ALDH4A1 antibody WB image
Western blot: 25 µg of Mouse skeletal muscle and BT474 cell lysates stained with ARG59775 anti-ALDH4A1 antibody at 1:1000 dilution.
