ARG63905
anti-ALDH1A1 antibody
anti-ALDH1A1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Developmental Biology antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ALDH1A1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ALDH1A1 |
| Antigen Species | Human |
| Immunogen | C-RELGEYGFHEYTE |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-S-53e; RalDH1; Aldehyde dehydrogenase family 1 member A1; Retinal dehydrogenase 1; HEL-9; RALDH 1; ALDH1; Aldehyde dehydrogenase, cytosolic; ALDH11; EC 1.2.1.36; RALDH1; HEL12; PUMB1; ALHDII; ALDC; ALDH-E1 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011] |
| Research Area | Cancer antibody; Developmental Biology antibody; Neuroscience antibody |
| Calculated MW | 55 kDa |
| PTM | The N-terminus is blocked most probably by acetylation. |
Images (3) Click the Picture to Zoom In
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ARG63905 anti-ALDH1A1 antibody WB image
Western Blot: Human Liver Lysate (35 µg protein in RIPA buffer) stained with ARG63905 anti-ALDH1A1 antibody at 1 µg/ml dilution.
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ARG63905 anti-ALDH1A1 antibody WB image
Western Blot: 1). Mock transfection; 2) ALDH1A1 expressing plasmid transfected HEK293 cell lysate standed with ARG63905 anti-ALDH1A1 antibody.
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ARG63905 anti-ALDH1A1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63905 anti-ALDH1A1 antibody at 5 µg/ml dilution followed by AP-staining.
