ARG41794

anti-AKAP14 antibody

anti-AKAP14 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes AKAP14
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AKAP14
Antigen Species Human
Immunogen Synthetic peptide of Human AKAP14.
Conjugation Un-conjugated
Alternate Names AKAP-14; Protein kinase A-anchoring protein 14; A-kinase anchor protein 28 kDa; A-kinase anchor protein 14; AKAP28; PRKA14; AKAP 28

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat
Observed Size ~ 21 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 158798 Human AKAP14

GeneID: 60332 Rat AKAP14

Swiss-port # O35817 Rat A-kinase anchor protein 14

Swiss-port # Q86UN6 Human A-kinase anchor protein 14

Gene Symbol AKAP14
Gene Full Name A kinase (PRKA) anchor protein 14
Background The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Function Binds to type II regulatory subunits of protein kinase A and anchors/targets them. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 23 kDa

Images (1) Click the Picture to Zoom In

  • ARG41794 anti-AKAP14 antibody WB image

    Western blot: Jurkat cell lysate stained with ARG41794 anti-AKAP14 antibody.