ARG57175
anti-AK1 antibody [7E9]
anti-AK1 antibody [7E9] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [7E9] recognizes AK1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7E9 |
| Isotype | IgG1, kappa |
| Target Name | AK1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-194 of Human AK1 |
| Conjugation | Un-conjugated |
| Alternate Names | Myokinase; Adenylate kinase isoenzyme 1; ATP-AMP transphosphorylase 1; ATP:AMP phosphotransferase; EC 2.7.4.6; HTL-S-58j; EC 2.7.4.3; Adenylate monophosphate kinase; AK 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AK1 |
| Gene Full Name | adenylate kinase 1 |
| Background | Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. [UniProt] |
| Calculated MW | 22 kDa |
Images (1) Click the Picture to Zoom In
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ARG57175 anti-AK1 antibody [7E9] WB image
Western blot: 40 µg of 1) HeLa, 2) U87-MG, 3) K562, 4) 293T, 5) HepG2, 6) A549, 7) MCF7, 8) SK-OV-3, and 9) PC3 cell lysates stained with ARG57175 anti-AK1 antibody [7E9] at 1:1000.
