ARG42926
anti-AIMP2 antibody
anti-AIMP2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AIMP2 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AIMP2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 298-320 of Human AIMP2. (NVQRWMRSCENLAPFNTALKLLK) |
| Conjugation | Un-conjugated |
| Alternate Names | PRO0992; JTV-1; JTV1; P38; Protein JTV-1; Multisynthase complex auxiliary component p38; Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 36 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13155 Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 Swiss-port # Q32PX2 Rat Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 |
|---|---|
| Gene Symbol | AIMP2 |
| Gene Full Name | aminoacyl tRNA synthetase complex-interacting multifunctional protein 2 |
| Background | The protein encoded by this gene is part of the aminoacyl-tRNA synthetase complex, which contains nine different aminoacyl-tRNA synthetases and three non-enzymatic factors. The encoded protein is one of the non-enzymatic factors and is required for assembly and stability of the complex. [provided by RefSeq, May 2016] |
| Function | Required for assembly and stability of the aminoacyl-tRNA synthase complex (PubMed:19131329). Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. Nucleus. Note=Following DNA damage, dissociates from the aminoacyl-tRNA synthase complex and translocates from the cytoplasm to the nucleus. [UniProt] |
| Calculated MW | 35 kDa |
| PTM | Phosphorylated on serine residues in response to UV irradiation. Ubiquitinated by PRKN, leading to its degradation by the proteasome. Mutant PRKN fails to ubiquitinate AIMP2 efficiently, allowing its accumulation which may contribute to neurodegeneration associated with Parkinson disease. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG42926 anti-AIMP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42926 anti-AIMP2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42926 anti-AIMP2 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat liver, Rat lung, Rat kidney, Rat brain, Jurkat, CEM, HUT and U93T cell lysates stained with ARG42926 anti-AIMP2 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42926 anti-AIMP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42926 anti-AIMP2 antibody at 1 µg/ml dilution, overnight at 4°C.
