ARG41233
anti-AIM2 antibody
anti-AIM2 antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes AIM2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AIM2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human AIM2. (C-DKQYKSVTKPKPLSQ) (NP_004824.1) |
| Conjugation | Un-conjugated |
| Alternate Names | PYHIN4; Interferon-inducible protein AIM2; Absent in melanoma 2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 39 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AIM2 |
| Gene Full Name | absent in melanoma 2 |
| Background | AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq, Jul 2008] |
| Function | Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Activated inflammasomes can aggregate in the cytosol as speck-like particles. [UniProt] |
| Calculated MW | 39 kDa |
Images (1) Click the Picture to Zoom In
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ARG41233 anti-AIM2 antibody WB image
Western blot: 35 µg of Daudi cell lysate (in RIPA buffer) stained with ARG41233 anti-AIM2 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
