ARG66564
anti-AGTR1 / AT1 antibody
anti-AGTR1 / AT1 antibody for ICC/IF,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AGTR1 / AT1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AGTR1 / AT1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of Human AT1. |
| Conjugation | Un-conjugated |
| Alternate Names | AT1AR; Angiotensin II type-1 receptor; AT1R; Type-1 angiotensin II receptor; AT1BR; AG2S; AT2R1; HAT1R; AT1; AGTR1B; AT1B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 41 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AGTR1 |
| Gene Full Name | angiotensin II receptor, type 1 |
| Background | Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012] |
| Function | Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. [UniProt] |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 41 kDa |
| PTM | C-terminal Ser or Thr residues may be phosphorylated. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66564 anti-AGTR1 / AT1 antibody ICC/IF image
Immunofluorescence: Formalin-fixed K562 cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66564 anti-AGTR1 / AT1 antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. DAPI was used to stain the cell nuclei (blue).
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ARG66564 anti-AGTR1 / AT1 antibody WB image
Western blot: HepG2, K562 and RAW264.7 whole cell lysates stained with ARG66564 anti-AGTR1 / AT1 antibody.
