ARG59686
anti-AGFG1 antibody
anti-AGFG1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AGFG1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AGFG1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 5-28 of Human AGFG1. (AKRKQEEKHLKMLRDMTGLPHNRK) |
| Conjugation | Un-conjugated |
| Alternate Names | Rev/Rex activation domain-binding protein; RAB; Rev-interacting protein; HIV-1 Rev-binding protein; RIP; Nucleoporin-like protein RIP; Arf-GAP domain and FG repeat-containing protein 1; HRB |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P52594 Human Arf-GAP domain and FG repeat-containing protein 1 Swiss-port # Q4KLH5 Rat Arf-GAP domain and FG repeat-containing protein 1 |
|---|---|
| Gene Symbol | AGFG1 |
| Gene Full Name | ArfGAP with FG repeats 1 |
| Background | The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
| Function | Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasmic vesicle. [UniProt] |
| Calculated MW | 58 kDa |
| PTM | O-glycosylated. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG59686 anti-AGFG1 antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG59686 anti-AGFG1 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG59686 anti-AGFG1 antibody WB image
Western blot: 50 µg of Rat testis, 50 µg of Rat liver, 50 µg of Human placenta, 40 µg of MCF-7 whole cell lysate and 40 µg of HeLa whole cell lysate stained with ARG59686 anti-AGFG1 antibody at 0.5 µg/ml dilution.
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ARG59686 anti-AGFG1 antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG59686 anti-AGFG1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
