ARG57017

anti-ADI1 antibody [27E8]

anti-ADI1 antibody [27E8] for Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [27E8] recognizes ADI1
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 27E8
Isotype IgG1, kappa
Target Name ADI1
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-179 of Human ADI1.
Conjugation Un-conjugated
Alternate Names 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; mtnD; SIPL; Fe-ARD; APL1; MTCBP1; Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1; Sip-L; EC 1.13.11.54; Submergence-induced protein-like factor; MTCBP-1; ARD; Fe; 2+; Acireductone dioxygenase; Ni-ARD; HMFT1638

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55256 Human ADI1

Swiss-port # Q9BV57 Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase

Gene Symbol ADI1
Gene Full Name acireductone dioxygenase 1
Background This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Function Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line. [UniProt]
Calculated MW 21 kDa

Images (1) Click the Picture to Zoom In

  • ARG57017 anti-ADI1 antibody [27E8] WB image

    Western blot: 40 µg of HepG2 cell lysate stained with ARG57017 anti-ADI1 antibody [27E8] at 1) 1:500, 2) 1:1000, and, 3) 1:5000 dilution.