ARG57017
anti-ADI1 antibody [27E8]
anti-ADI1 antibody [27E8] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [27E8] recognizes ADI1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 27E8 |
| Isotype | IgG1, kappa |
| Target Name | ADI1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-179 of Human ADI1. |
| Conjugation | Un-conjugated |
| Alternate Names | 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; mtnD; SIPL; Fe-ARD; APL1; MTCBP1; Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1; Sip-L; EC 1.13.11.54; Submergence-induced protein-like factor; MTCBP-1; ARD; Fe; 2+; Acireductone dioxygenase; Ni-ARD; HMFT1638 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BV57 Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase |
|---|---|
| Gene Symbol | ADI1 |
| Gene Full Name | acireductone dioxygenase 1 |
| Background | This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
| Function | Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line. [UniProt] |
| Calculated MW | 21 kDa |
Images (1) Click the Picture to Zoom In
-
ARG57017 anti-ADI1 antibody [27E8] WB image
Western blot: 40 µg of HepG2 cell lysate stained with ARG57017 anti-ADI1 antibody [27E8] at 1) 1:500, 2) 1:1000, and, 3) 1:5000 dilution.
