ARG43632
anti-ADAM9 antibody
anti-ADAM9 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit polyclonal antibody recognizes ADAM9 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ADAM9 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment corresponding to a.a. R212-D247 of Human ADAM9. |
| Conjugation | Un-conjugated |
| Protein Full Name | Disintegrin and metalloproteinase domain-containing protein 9 |
| Alternate Names | MDC9; Meltrin-gamma; ADAM 9; Metalloprotease/disintegrin/cysteine-rich protein 9; Disintegrin and metalloproteinase domain-containing protein 9; Cellular disintegrin-related protein; EC 3.4.24.-; CORD9; Mltng; MCMP; Myeloma cell metalloproteinase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | A431, U87-MG | ||||||||
| Observed Size | ~ 70-80 kDa (mature form), 100-115 kDa (pro-form) |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13443 Human Disintegrin and metalloproteinase domain-containing protein 9 |
|---|---|
| Gene Symbol | ADAM9 |
| Gene Full Name | ADAM metallopeptidase domain 9 |
| Background | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010] |
| Function | Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. [UniProt] |
| Cellular Localization | Cell membrane, Membrane, Secreted |
| Calculated MW | 90.5 kDa |
| PTM | Proteolytically cleaved in the trans-Golgi network before it reaches the plasma membrane to generate a mature protein. The removal of the pro-domain occurs via cleavage at two different sites. Processed most likely by a pro-protein convertase such as furin, at the boundary between the pro-domain and the catalytic domain. An additional upstream cleavage pro-protein convertase site (Arg-56/Glu-57) has an important role in the activation of ADAM9. Phosphorylation is induced in vitro by phorbol-12-myristate-13-acetate (PMA). [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG43632 anti-ADAM9 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human uterus tissue stained with ARG43632 anti-ADAM9 antibody at 1:100 dilution. Antigen Retrieval: Heat mediated was performed using EDTA buffer (pH 8.0).
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ARG43632 anti-ADAM9 antibody ICC/IF image
Immunofluorescence: U20S cells were blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG43632 anti-ADAM9 antibody at 1:100 and for 30 minutes at 37°C.
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ARG43632 anti-ADAM9 antibody WB image
Western blot: A431 cell lysates stained with ARG43632 anti-ADAM9 antibody at 1:1500 dilution.
