ARG63730
anti-ADAM12 antibody
anti-ADAM12 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ADAM12 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P |
| Specificity | This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ADAM12 |
| Antigen Species | Human |
| Immunogen | AARPLPVSPARALC |
| Conjugation | Un-conjugated |
| Alternate Names | MCMP; ADAM 12; MCMPMltna; EC 3.4.24.-; Disintegrin and metalloproteinase domain-containing protein 12; Meltrin-alpha; MLTN; MLTNA; CAR10; ADAM12-OT1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43184 Human Disintegrin and metalloproteinase domain-containing protein 12 |
|---|---|
| Background | This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 100 kDa |
| PTM | The precursor is cleaved by a furin endopeptidase. |
Images (4) Click the Picture to Zoom In
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ARG63730 anti-ADAM12 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63730 anti-ADAM12 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63730 anti-ADAM12 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63730 anti-ADAM12 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63730 anti-ADAM12 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Prostate. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63730 anti-ADAM12 antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG63730 anti-ADAM12 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63730 anti-ADAM12 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
