ARG58576
anti-ACSL3 antibody
anti-ACSL3 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACSL3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACSL3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to M12-I224 of Human ACSL3. |
| Conjugation | Un-conjugated |
| Alternate Names | PRO2194; ACS3; Long-chain-fatty-acid--CoA ligase 3; FACL3; Long-chain acyl-CoA synthetase 3; EC 6.2.1.3; LACS 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95573 Human Long-chain-fatty-acid--CoA ligase 3 |
|---|---|
| Gene Symbol | ACSL3 |
| Gene Full Name | acyl-CoA synthetase long-chain family member 3 |
| Background | The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). [UniProt] |
| Cellular Localization | Mitochondrion outer membrane; Single-pass type III membrane protein. Peroxisome membrane; Single-pass type III membrane protein. Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein. [UniProt] |
| Calculated MW | 80 kDa |
Images (1) Click the Picture to Zoom In
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ARG58576 anti-ACSL3 antibody WB image
Western blot: HepG2 whole cell lysate stained with ARG58576 anti-ACSL3 antibody at 0.5 µg/ml dilution.
