ARG65247

anti-ACPP antibody

anti-ACPP antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody

Overview

Product Description Goat Polyclonal antibody recognizes ACPP
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog
Tested Application ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ACPP
Antigen Species Human
Immunogen C-YGIHKQKEKSRLQ
Conjugation Un-conjugated
Alternate Names PAP; ACP-3; TMPase; 5'-nucleotidase; ACP3; EC 3.1.3.5; Ecto-5'-nucleotidase; Thiamine monophosphatase; EC 3.1.3.2; 5'-NT; Prostatic acid phosphatase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-P3 - 5 µg/ml
WB0.3 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human prostate
Observed Size ~ 50 kDa

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55 Human ACPP

GeneID: 56318 Mouse ACPP

Swiss-port # P15309 Human Prostatic acid phosphatase

Swiss-port # Q8CE08 Mouse Prostatic acid phosphatase

Background ACPP is an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]
Function ACPP is a non-specific tyrosine phosphatase. It dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.

Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody
Calculated MW 45 kDa
PTM N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides.
Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection) which entrap HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection.

Images (2) Click the Picture to Zoom In

  • ARG65247 anti-ACPP antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65247 anti-ACPP antibody at 3.8 µg/ml dilution, followed by AP-staining.

  • ARG65247 anti-ACPP antibody WB image

    Western blot: 35 µg of Human prostate lysate (in RIPA buffer) stained with ARG65247 anti-ACPP antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.