ARG57118
anti-ACAT1 antibody [15E5]
anti-ACAT1 antibody [15E5] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [15E5] recognizes ACAT1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 15E5 |
| Isotype | IgG1, kappa |
| Target Name | ACAT1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 34-427 of Human ACAT1 |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.3.1.9; Acetoacetyl-CoA thiolase; MAT; T2; ACAT; THIL; Acetyl-CoA acetyltransferase, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P24752 Human Acetyl-CoA acetyltransferase, mitochondrial |
|---|---|
| Gene Symbol | ACAT1 |
| Gene Full Name | acetyl-CoA acetyltransferase 1 |
| Background | This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009] |
| Function | Plays a major role in ketone body metabolism. [UniProt] |
| Calculated MW | 45 kDa |
| PTM | Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity). |
Images (3) Click the Picture to Zoom In
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ARG57118 anti-ACAT1 antibody [15E5] ICC/IF image
Immunofluorescence: Hep3B cells line stained with ARG57118 anti-ACAT1 antibody [15E5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57118 anti-ACAT1 antibody [15E5] WB image
Western blot: 40 µg of HepG2 and HeLa cell lysates stained with ARG57118 anti-ACAT1 antibody [15E5] at 1:1000.
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ARG57118 anti-ACAT1 antibody [15E5] WB image
Western blot: 40 µg of 1) Hep3B, 2) 293T, and 3) A431 cell lysates stained with ARG57118 anti-ACAT1 antibody [15E5] at 1:1000.
