ARG43149
anti-ACADS antibody
anti-ACADS antibody for Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACADS |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACADS |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-260 of Human ACADS (NP_000008.1). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 1.3.8.1; Butyryl-CoA dehydrogenase; ACAD3; SCAD; Short-chain specific acyl-CoA dehydrogenase, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HepG2 | ||||||
| Observed Size | ~ 42 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACADS |
| Gene Full Name | acyl-CoA dehydrogenase, C-2 to C-3 short chain |
| Background | This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014] |
| Function | Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains (PubMed:21237683, PubMed:11134486). [UniProt] |
| Cellular Localization | Mitochondrion matrix. [UniProt] |
| Calculated MW | 44 kDa |
Images (2) Click the Picture to Zoom In
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ARG43149 anti-ACADS antibody WB image
Western blot: 25 µg of HepG2 cell lysate stained with ARG43149 anti-ACADS antibody at 1:1000 dilution.
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ARG43149 anti-ACADS antibody IP image
Immunoprecipitation: 200 µg extracts of MCF7 cells were immunoprecipitated and stained with ARG43149 anti-ACADS antibody at 1:1000 dilution.
