ARG66308

anti-ACAA2 antibody

anti-ACAA2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes ACAA2
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ACAA2
Antigen Species Human
Immunogen Fusion protein of Human ACAA2.
Conjugation Un-conjugated
Alternate Names Acetyl-CoA acyltransferase; Beta-ketothiolase; T1; EC 2.3.1.16; DSAEC; 3-ketoacyl-CoA thiolase, mitochondrial; Mitochondrial 3-oxoacyl-CoA thiolase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:25 - 1:100
WB1:200 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: NIH/3T3 and A172 cells, Human liver cancer tissue.
IHC-P: Human colon cancer and Human thyroid cancer.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol.
Preservative 0.05% Sodium azide
Stabilizer 40% Glycerol
Concentration 1.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10449 Human ACAA2

GeneID: 52538 Mouse ACAA2

Swiss-port # P42765 Human 3-ketoacyl-CoA thiolase, mitochondrial

Swiss-port # Q8BWT1 Mouse 3-ketoacyl-CoA thiolase, mitochondrial

Gene Symbol ACAA2
Gene Full Name acetyl-CoA acyltransferase 2
Background The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. [provided by RefSeq, Jul 2008]
Function Abolishes BNIP3-mediated apoptosis and mitochondrial damage. [UniProt]
Calculated MW 42 kDa

Images (3) Click the Picture to Zoom In

  • ARG66308 anti-ACAA2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG66308 anti-ACAA2 antibody (left) at 1:30 dilution, or the same antibody pre-incubated with antigen (right). (Original magnification: X200) .

  • ARG66308 anti-ACAA2 antibody WB image

    Western blot: 40 µg of NIH/3T3, A172 and Human liver cancer lysates stained with ARG66308 anti-ACAA2 antibody at 1:375 dilution.

  • ARG66308 anti-ACAA2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG66308 anti-ACAA2 antibody (left) at 1:30 dilution, or the same antibody pre-incubated with antigen (right). (Original magnification: X200) .