ARG66193
anti-ABCG2 antibody
anti-ABCG2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ABCG2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Specificity | The antibody detects endogenous ABCG2 protein. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ABCG2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 461-510 of Human ABCG2. |
| Conjugation | Un-conjugated |
| Alternate Names | BCRP1; Urate exporter; BCRP; MRX; Placenta-specific ATP-binding cassette transporter; EST157481; GOUT1; MXR; Mitoxantrone resistance-associated protein; CDw338; CD338; ATP-binding cassette sub-family G member 2; ABC15; ABCP; UAQTL1; Breast cancer resistance protein; MXR1; MXR-1; BMDP; CD antigen CD338 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q7TMS5 Mouse ATP-binding cassette sub-family G member 2 Swiss-port # Q9UNQ0 Human ATP-binding cassette sub-family G member 2 |
|---|---|
| Gene Symbol | ABCG2 |
| Gene Full Name | ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group) |
| Background | The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| Function | High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. Implicated in the efflux of numerous drugs and xenobiotics: mitoxantrone, the photosensitizer pheophorbide, camptothecin, methotrexate, azidothymidine (AZT), and the anthracyclines daunorubicin and doxorubicin. [UniProt] |
| Calculated MW | 72 kDa |
| PTM | Glycosylation-deficient ABCG2 is normally expressed and functional. |
Images (3) Click the Picture to Zoom In
-
ARG66193 anti-ABCG2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66193 anti-ABCG2 antibody at 1:100 dilution.
-
ARG66193 anti-ABCG2 antibody WB image
Western blot: NIH-3T3 and 293 cell lysates stained with ARG66193 anti-ABCG2 antibody at 1:500 dilution.
-
ARG66193 anti-ABCG2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66193 anti-ABCG2 antibody 1:100 dilution.
