Overview

Product Description	Rabbit Polyclonal antibody recognizes ABCG2
Tested Reactivity	Hu
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	ABCG2
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human ABCG2.
Conjugation	Un-conjugated
Alternate Names	BCRP1; Urate exporter; BCRP; MRX; Placenta-specific ATP-binding cassette transporter; EST157481; GOUT1; MXR; Mitoxantrone resistance-associated protein; CDw338; CD338; ATP-binding cassette sub-family G member 2; ABC15; ABCP; UAQTL1; Breast cancer resistance protein; MXR1; MXR-1; BMDP; CD antigen CD338

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 9429 Human ABCG2 Swiss-port # Q9UNQ0 Human ATP-binding cassette sub-family G member 2
Gene Symbol	ABCG2
Gene Full Name	ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group)
Background	The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Function	High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. Implicated in the efflux of numerous drugs and xenobiotics: mitoxantrone, the photosensitizer pheophorbide, camptothecin, methotrexate, azidothymidine (AZT), and the anthracyclines daunorubicin and doxorubicin. [UniProt]
Cellular Localization	Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. [UniProt]
Calculated MW	72 kDa
PTM	Glycosylation-deficient ABCG2 is normally expressed and functional. [UniProt]

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