ARG40552
anti-ABCG2 antibody
anti-ABCG2 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ABCG2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ABCG2 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 260-395 of Human ABCG2 (NP_004818.2). |
| Conjugation | Un-conjugated |
| Alternate Names | BCRP1; Urate exporter; BCRP; MRX; Placenta-specific ATP-binding cassette transporter; EST157481; GOUT1; MXR; Mitoxantrone resistance-associated protein; CDw338; CD338; ATP-binding cassette sub-family G member 2; ABC15; ABCP; UAQTL1; Breast cancer resistance protein; MXR1; MXR-1; BMDP; CD antigen CD338 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | COS7 | ||||||
| Observed Size | 70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ABCG2 |
| Gene Full Name | ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group) |
| Background | The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| Function | High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. Implicated in the efflux of numerous drugs and xenobiotics: mitoxantrone, the photosensitizer pheophorbide, camptothecin, methotrexate, azidothymidine (AZT), and the anthracyclines daunorubicin and doxorubicin. [UniProt] |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 72 kDa |
| PTM | Glycosylation-deficient ABCG2 is normally expressed and functional. [UniProt] |
