ARG64411
anti-ABCB5 antibody
anti-ABCB5 antibody for Flow cytometry,Western blot and Human
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ABCB5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1) |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ABCB5 |
| Antigen Species | Human |
| Immunogen | C-QTQHRNTSKKAQ |
| Conjugation | Un-conjugated |
| Alternate Names | ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5; ABCB5beta; EST422562; ABCB5alpha |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q2M3G0 Human ATP-binding cassette sub-family B member 5 |
|---|---|
| Background | ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 139 kDa |
Images (2) Click the Picture to Zoom In
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ARG64411 anti-ABCB5 antibody WB image
Western Blot: A431 cell lysate (35 µg protein in RIPA buffer). stained with ARG64411 anti-ABCB5 antibody at 0.3 µg/ml dilution.
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ARG64411 anti-ABCB5 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG64411 anti-ABCB5 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
