ARG23548
anti-ABCA1 antibody [5A1-1422] (FITC)
anti-ABCA1 antibody [5A1-1422] (FITC) for Flow cytometry and Mouse
Overview
| Product Description | FITC-conjugated Rat Monoclonal antibody [5A1-1422] recognizes ABCA1. Rat anti Mouse ABCA1 antibody, clone 5A1-1422 recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1). The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease.Mutations in ABCA1 gene have been associated with Tangiers disease, a genetic disorder of lipid metabolism, and familial high density lipoprotein (HDL) deficiency. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 5A1-1422 |
| Isotype | IgG2a |
| Target Name | ABCA1 |
| Antigen Species | Mouse |
| Immunogen | ABCA1 transfected HeLa cells. |
| Conjugation | FITC |
| Alternate Names | TGD; Cholesterol efflux regulatory protein; HDLDT1; ATP-binding cassette sub-family A member 1; ATP-binding cassette 1; ATP-binding cassette transporter 1; ABC-1; ABC1; CERP |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide and 1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P41233 Mouse ATP-binding cassette sub-family A member 1 |
|---|---|
| Gene Symbol | ABCA1 |
| Gene Full Name | ATP-binding cassette, sub-family A (ABC1), member 1 |
| Background | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008] |
| Function | cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport. [UniProt] |
| Calculated MW | 254 kDa |
| PTM | Phosphorylation on Ser-2054 regulates phospholipid efflux. Palmitoylation by DHHC8 is essential for membrane localization. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG23548 anti-ABCA1 antibody [5A1-1422] (FITC) FACS image
Flow Cytometry: pCPT-cAMP stimulated RAW264.7 cells stained with ARG23548 anti-ABCA1 antibody [5A1-1422] (FITC).
