ARG52244
anti-AATF phospho (Ser477) antibody
anti-AATF phospho (Ser477) antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AATF phospho (Ser477) |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Bov, Dog, NHuPrm, Sheep, Xenopus laevis |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AATF |
| Antigen Species | Human |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | BFR2; Apoptosis-antagonizing transcription factor; CHE1; CHE-1; Rb-binding protein Che-1; Protein AATF; DED |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | Specific for the ~66k Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to geneRate the antibody but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AATF |
| Gene Full Name | apoptosis antagonizing transcription factor |
| Background | Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006). |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody |
| Calculated MW | 63 kDa |
| PTM | Hyperphosphorylated during the G1/S phase transition. |
Images (1) Click the Picture to Zoom In
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ARG52244 anti-AATF phospho (Ser477) antibody WB image
Western blot: HeLa lysate showing specific immunolabeling of the ~66k Che-1 protein phosphorylated at Ser 477 stained with ARG52244 anti-AATF phospho (Ser477) antibody.
The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen.
