ARG65033
anti-AAK1 antibody
anti-AAK1 antibody for Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes AAK1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AAK1 |
| Antigen Species | Human |
| Immunogen | C-GSPRTSQQNVYNPSE |
| Conjugation | Un-conjugated |
| Alternate Names | AP2-associated protein kinase 1; EC 2.7.11.1; Adaptor-associated kinase 1 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 104 kDa |
| PTM | Autophosphorylated. |
Images (1) Click the Picture to Zoom In
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ARG65033 anti-AAK1 antibody WB image
Western Blot: Human Amygdala lysate (35 µg protein in RIPA buffer) stained with ARG65033 anti-AAK1 antibody at 1 µg/ml dilution.
