ARG57359

anti-A RAF antibody

anti-A RAF antibody for Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes A RAF
Tested Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name A RAF
Antigen Species Human
Immunogen Recombinant Protein of Human A-RAF.
Conjugation Un-conjugated
Alternate Names Proto-oncogene A-Raf; ARAF1; Proto-oncogene A-Raf-1; RAFA1; PKS2; Proto-oncogene Pks; A-RAF; EC 2.7.11.1; Serine/threonine-protein kinase A-Raf

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 369 Human ARAF

Swiss-port # P10398 Human Serine/threonine-protein kinase A-Raf

Gene Symbol ARAF
Gene Full Name A-Raf proto-oncogene, serine/threonine kinase
Background This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.

Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation. [UniProt]
Calculated MW 68 kDa

Images (1) Click the Picture to Zoom In

  • ARG57359 anti-A RAF antibody WB image

    Western blot: MCF7 cell lysate stained with ARG57359 anti-A RAF antibody.