ARG64018
anti-5-HT3A Receptor antibody
anti-5-HT3A Receptor antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes 5-HT3A Receptor |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognise all three reported isoforms (NP_998786.2; NP_000860.2; NP_001155244.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | 5-HT3A Receptor |
| Antigen Species | Human |
| Immunogen | C-GPQDFEKSPRDR |
| Conjugation | Un-conjugated |
| Alternate Names | 5-HT-3; 5-hydroxytryptamine receptor 3; Serotonin-gated ion channel receptor; 5-HT3A; 5-HT3-A; HTR3; 5HT3R; Serotonin receptor 3A; 5-hydroxytryptamine receptor 3A; 5-HT3R |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Research Area | Metabolism antibody; Neuroscience antibody |
| Calculated MW | 55 kDa |
Images (2) Click the Picture to Zoom In
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ARG64018 anti-5-HT3A Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64018 anti-5-HT3A Receptor antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64018 anti-5-HT3A Receptor antibody WB image
Western blot: 35 µg of Human Colon lysate (in RIPA buffer) stained with ARG64018 anti-5-HT3A Receptor antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
