ARG63766
anti-5-HT2C Receptor antibody
anti-5-HT2C Receptor antibody for Western blot and Human
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes 5-HT2C Receptor |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | 5-HT2C Receptor |
| Antigen Species | Human |
| Immunogen | C-QVENLELPVN |
| Conjugation | Un-conjugated |
| Alternate Names | 5-hydroxytryptamine receptor 1C; 5-HT-1C; 5-HTR2C; 5-HT2C; 5-HT-2C; 5-HT1C; Serotonin receptor 2C; 5HTR2C; 5-hydroxytryptamine receptor 2C; HTR1C |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 52 kDa |
| PTM | N-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG63766 anti-5-HT2C Receptor antibody WB image
Western blot: 35 µg of EBV immortalised Lymphoblastoid lysate (in RIPA buffer) stained with ARG63766 anti-5-HT2C Receptor antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
