ARG55889
anti-5-HT / Serotonin antibody [YC5/45]
anti-5-HT / Serotonin antibody [YC5/45] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and All species,Human,Mouse,Rat
Overview
| Product Description | Rat Monoclonal antibody [YC5/45] recognizes 5-HT / Serotonin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | ICC/IF, IHC-Fr, IHC-P |
| Specificity | The antibody specifically recognizes the formaldehyde conjugate of serotonin. Without the paraformaldehyde treatment, the antibody cross-reacts with dopamine and tryptamine in liquid phase haemagglutination tests. It does not react with catecholamine-containing neurons in formaldehyde fixed preparations. The antibody reacts with serotonin in neurons and recognises serotonergic sites in fixed tissue sections. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | YC5/45 |
| Isotype | IgG |
| Target Name | 5-HT / Serotonin |
| Conjugation | Un-conjugated |
| Alternate Names | 5-hydroxytryptamine; 5-HT |
Application Instructions
| Application Suggestion |
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| Application Note | Antibody reactivity and working conditions may vary between species. IHC-P: boiling the sections in Tris/EDTA-buffer (pH 9.0) IHC-Fr: formaldehyde or paraformaldehyde-fixed tissue is recommended. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Non Purified |
| Buffer | Tissue culture supernatants and 0.05% Thimerosal |
| Preservative | 0.05% Thimerosal |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
