ARG23717
anti-4 Hydroxynonenal antibody [12F7]
anti-4 Hydroxynonenal antibody [12F7] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Other
Ferroptosis/Oxytosis Study antibody; Lipid peroxidation Marker antibody
Publication8
Overview
| Product Description | Mouse Monoclonal antibody [12F7] recognizes 4 Hydroxynonenal |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ELISA, ICC/IF, IHC-P, WB |
| Specificity | Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 12F7 |
| Isotype | IgG1 |
| Target Name | 4 Hydroxynonenal |
| Antigen Species | Others |
| Immunogen | Synthetic 4-Hydroxynonenal modified KLH. |
| Conjugation | Un-conjugated |
| Alternate Names | 4-HNE |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Highlight | Related Antibody Duos and Panels: ARG30337 Ferroptosis / Oxytosis Antibody Panel Related products: Hydroxynonenal antibodies; Hydroxynonenal ELISA Kits; Hydroxynonenal Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Ferroptosis/Oxytosis Antibody Panel is launched Pericytes, new therapeutic target for Alzheimer's disease? Therapeutic strategies against PDAC |
|---|---|
| Research Area | Ferroptosis/Oxytosis Study antibody; Lipid peroxidation Marker antibody |
Images (3) Click the Picture to Zoom In
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ARG23717 anti-4 Hydroxynonenal antibody [12F7] IHC-P image
Immunohistochemistry: Mouse testis stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:50 dilution.
From Lipeng Li et al. FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice (2023), doi: 10.1016/j.ecoenv.2023.115309, Fig. 4A1.
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ARG23717 anti-4 Hydroxynonenal antibody [12F7] ICC/IF image
Immunofluorescence: HEK293 cells fixed with 5% Formaldehyde (5 min). Cells were stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:50 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G): Untreated. (B,D,F,H): Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibodies. (G,H) Composite.
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ARG23717 anti-4 Hydroxynonenal antibody [12F7] WB image
Western blot: 0.5 µg of BSA, 0.5 µg of 4-hydroxyl nonenal-BSA, 2.0 µg of 4-hydroxy nonenal-BSA, 0.5 µg of 4-hydroxy-2-hexenal and 2.0 µg of 4-hydroxy-2-hexenal stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:1000 for 2 hours at RT.
Specific References
Effects of liraglutide on astrocyte polarization and neuroinflammation in db/db mice: focus on iron overload and oxidative stress
IHC-P / Mouse
Mechanism of YY1 mediating autophagy dependent ferroptosis in PM2.5 induced cardiac fibrosis
IHC-P / Mouse
Hydrogen Attenuates Chronic Intermittent Hypoxia-Induced Cardiac Hypertrophy by Regulating Iron Metabolism
WB / Mouse
FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice
IHC-P / Mouse
A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage
WB / Human
Fermented and Aged Ginseng Sprouts ( Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis
WB / Mouse
Liraglutide attenuates hepatic iron levels and ferroptosis in db/db mice
WB / Mouse
Oyster-Derived Tyr-Ala (YA) Peptide Prevents Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure by Suppressing Inflammatory, Apoptotic, Ferroptotic, and Pyroptotic Signals
WB / Mouse
