Overview

Product Description	Rabbit Polyclonal antibody recognizes 14-3-3 (pan)
Tested Reactivity	Hu, Ms, Rat
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	14-3-3 (pan)
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human 14-3-3.
Conjugation	Un-conjugated
Alternate Names	14-3-3; Protein HS1; 1C5; HS1; 14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:5000 - 1:20000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 10971 Human YWHAQ GeneID: 22630 Mouse YWHAQ GeneID: 25577 Rat YWHAQ Swiss-port # P27348 Human 14-3-3 protein theta Swiss-port # P68254 Mouse 14-3-3 protein theta Swiss-port # P68255 Rat 14-3-3 protein theta More
Gene Symbol	YWHAQ
Gene Full Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
Background	This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]
Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. [UniProt]
Cellular Localization	Cytoplasm. Note=In neurons, axonally transported to the nerve terminals. [UniProt]
Calculated MW	28 kDa
PTM	Ser-232 is probably phosphorylated by CK1. [UniProt]

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