ARG57137
anti-14-3-3 gamma antibody [4B9]
anti-14-3-3 gamma antibody [4B9] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [4B9] recognizes 14-3-3 gamma |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4B9 |
| Isotype | IgG1, kappa |
| Target Name | 14-3-3 gamma |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-247 of Human 14-3-3 gamma |
| Conjugation | Un-conjugated |
| Alternate Names | 14-3-3 protein gamma; PPP1R170; 14-3-3GAMMA; KCIP-1; Protein kinase C inhibitor protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | YWHAG |
| Gene Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma |
| Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008] |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. [UniProt] |
| Calculated MW | 28 kDa |
| PTM | Phosphorylated by various PKC isozymes. |
Images (4) Click the Picture to Zoom In
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ARG57137 anti-14-3-3 gamma antibody [4B9] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57137 anti-14-3-3 gamma antibody [4B9] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57137 anti-14-3-3 gamma antibody [4B9] WB image
Western blot: 40 µg of HeLa cell and Mouse brain lysates stained with ARG57137 anti-14-3-3 gamma antibody [4B9] at 1:1000.
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ARG57137 anti-14-3-3 gamma antibody [4B9] WB image
Western blot: 50 ng of Human 1) YWHAZ, 2) YWHAB, 3) YWHAE, 4) YWHAH, 5) YWHAG, 6) SFN, and 7) YWHAQ recombinant proteins stained with ARG57137 anti-14-3-3 gamma antibody [4B9] at 1:1000.
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ARG57137 anti-14-3-3 gamma antibody [4B9] WB image
Western blot: 40 µg of 1) 293T, 2) A431, 3) K562, and 4) NIH3T3 cell lysates stained with ARG57137 anti-14-3-3 gamma antibody [4B9] at 1:1000.
