ARG10690
anti-14-3-3 eta antibody [3G12]
anti-14-3-3 eta antibody [3G12] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Pig
Overview
| Product Description | Mouse Monoclonal antibody [3G12] recognizes 14-3-3 eta |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow, Pig |
| Predict Reactivity | Chk |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3G12 |
| Isotype | IgG1 |
| Target Name | 14-3-3 eta |
| Antigen Species | Human |
| Immunogen | Full length Human recombinant 14-3-3 eta protein. |
| Conjugation | Un-conjugated |
| Alternate Names | YWHA1; 14-3-3 protein eta; Protein AS1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | YWHAH |
| Gene Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta |
| Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009] |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. [UniProt] |
| Calculated MW | 28 kDa |
| PTM | Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. |
Images (3) Click the Picture to Zoom In
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ARG10690 anti-14-3-3 eta antibody [3G12] ICC/IF image
Immunocytochemistry: Rat mixed neuron / glial cell cultures stained with ARG10690 anti-14-3-3 eta antibody [3G12] (red). Neuronal perikarya are very rich in 14-3-3η which has a diffused cytoplasmic staining pattern. The blue is DAPI staining of nuclear DNA.
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ARG10690 anti-14-3-3 eta antibody [3G12] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG10690 anti-14-3-3 eta antibody [3G12] (red) at 1:1000 dilution. DAPI (blue) for nuclear staining.
Clone 3G12 reveals the diffuse cytoplasmic distribution of 14-3-3 eta protein with higher concentration in the perinuclear region.
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ARG10690 anti-14-3-3 eta antibody [3G12] WB image
Western blot: 1) protein standard, 2) Rat whole brain, 3) Mouse whole brain, 4) NIH/3T3, 5) Hek293, 6) HeLa, 7) SH-SY5Y, and 8) C6 cells stained with ARG10690 anti-14-3-3 eta antibody [3G12].
