ARG55210
anti-12 Lipoxygenase antibody
anti-12 Lipoxygenase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rabbit
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes 12 Lipoxygenase |
|---|---|
| Tested Reactivity | Hu, Ms, Rb |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | 12 Lipoxygenase |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 618-650 (C-terminus) of Human 12 Lipoxygenase. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 1.13.11.31; Platelet-type lipoxygenase 12; 12S-LOX; 12-LOX; 12S-lipoxygenase; Arachidonate 12-lipoxygenase, 12S-type; EC 3.3.2.-; LOG12; Lipoxin synthase 12-LO |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P18054 Human Arachidonate 12-lipoxygenase, 12S-type Swiss-port # P39655 Mouse Arachidonate 12-lipoxygenase, 12S-type |
|---|---|
| Gene Symbol | ALOX12 |
| Gene Full Name | arachidonate 12-lipoxygenase |
| Function | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 76 kDa |
Images (4) Click the Picture to Zoom In
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ARG55210 anti-12 Lipoxygenase antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG55210 anti-12 Lipoxygenase antibody (green) at 1:100 dilution. Cytoplasmic actin was counterstained with Dylight Fluor® 554 conjugated Phalloidin (red).
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ARG55210 anti-12 Lipoxygenase antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human skin tissue stained with ARG55210 anti-12 Lipoxygenase antibody.
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ARG55210 anti-12 Lipoxygenase antibody WB image
Western blot: 35 µg of K562 cell lysate stained with ARG55210 anti-12 Lipoxygenase antibody.
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ARG55210 anti-12 Lipoxygenase antibody FACS image
Flow Cytometry: K562 cells stained with ARG55210 anti-12 Lipoxygenase antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
