ARG82198

Xanthine Oxidase Activity Assay Kit (Colorimetric)

Xanthine Oxidase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All

Overview

Product Description ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric) can be used to measure Xanthine Oxidase activity in serum and cell lysate.
Tested Reactivity Hu, Ms, Rat, All
Tested Application FuncSt
Target Name Xanthine Oxidase
Conjugation Note Read at 570 nm (colorimetric), or 530/585 nm (fluorometric)
Sensitivity OD: 0.03 U/L; FL: 0.01 U/L
Detection Range 0.03 - 25 U/L (colorimetric); 0.01 - 2.5 U/L (fluorometric)
Sample Type serum and cell lysate
Sample Volume 10 µl
Alternate Names EC 1.17.1.4; XOR; XO; Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase; XD; EC 1.17.3.2; Xanthine oxidoreductase

Application Instructions

Application Note Please note that this kit does not include a microplate.
Assay Time 20 min

Properties

Form Liquid
Storage Instruction Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22436 Mouse XDH

GeneID: 497811 Rat XDH

GeneID: 7498 Human XDH

Gene Symbol XDH
Gene Full Name xanthine dehydrogenase
Background Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]
Function Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). [UniProt]
Cellular Localization Cytoplasm. Peroxisome. Secreted. [UniProt]
PTM Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).

Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric) enzyme kinetics graph

    Kinetics of 0 and 25 U/L Xanthine Oxidase reaction, using ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric).

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