ARG82198
Xanthine Oxidase Activity Assay Kit (Colorimetric)
Xanthine Oxidase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All species
Overview
| Product Description | ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric) can be used to measure Xanthine Oxidase activity in serum and cell lysate. |
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| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | FuncSt |
| Target Name | Xanthine Oxidase |
| Conjugation Note | Read at 570 nm (colorimetric), or 530/585 nm (fluorometric) |
| Sensitivity | OD: 0.03 U/L; FL: 0.01 U/L |
| Detection Range | 0.03 - 25 U/L (colorimetric); 0.01 - 2.5 U/L (fluorometric) |
| Sample Type | serum and cell lysate |
| Sample Volume | 10 µl |
| Alternate Names | EC 1.17.1.4; XOR; XO; Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase; XD; EC 1.17.3.2; Xanthine oxidoreductase |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | 20 min |
Properties
| Form | Liquid |
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| Storage Instruction | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | XDH |
| Gene Full Name | xanthine dehydrogenase |
| Background | Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014] |
| Function | Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). [UniProt] |
| Cellular Localization | Cytoplasm. Peroxisome. Secreted. [UniProt] |
| PTM | Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 and 25 U/L Xanthine Oxidase reaction, using ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric).
| Title | Download Link |
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| ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric) User manual |
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