ARG80817
Vitamin H (Biotin) ELISA Kit
Vitamin H (Biotin) ELISA Kit for ELISA and Other
Controls and Markers kit; Metabolism kit; Signaling Transduction kit
Overview
| Product Description | ARG80817 Vitamin H (Biotin) ELISA Kit is a Enzyme Immunoassay kit for the quantification of Vitamin H (Biotin) in food (extraction, dilution). |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ELISA |
| Target Name | Vitamin H |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.5 ng/ml |
| Sample Type | Food (extraction, dilution). |
| Standard Range | 1 - 25 ng/ml |
| Sample Volume | 50 µl |
Application Instructions
| Assay Time | 1 h (RT/shaker), 20 min (RT/dark) |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Vitamin H / Biotin |
|---|---|
| Background | Biotin serves as the prosthetic group of enzymes, which catalyze carboxylations in the organism. For this purpose, biotin is bound via its carboxy group to lysin residues of carboxylases, and the transfer of carbon dioxide takes place after its attachment to a nitrogen atom of biotin, forming the so-called active carbon dioxide. The awareness of the population for a good health and its interest in healthy nutrition has increased significantly during the last years. After the content of vitamins in his nourishment has gained importance for the consumer, food has partially been vitaminized by the manufacturer. When there exists a lack of biotin, seborrhoea, dermatitis, anorexia, muscle pain, tiredness and nervous disorders can appear. As biotin is synthesized by the human intestinal flora, deficiency symptoms are rare, appear however after excessive ingestion of raw egg white, which can be explained by its content of biotin-binding avidin. |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Controls and Markers kit; Metabolism kit; Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
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ARG80817 Vitamin H (Biotin) ELISA Kit standard curve image
ARG80817 Vitamin H (Biotin) ELISA Kit results of a typical standard run with optical density reading at 450nm.
| Title | Download Link |
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| ARG80817 Vitamin H (Biotin) ELISA Kit User's manual |
 Download
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