ARG80816
Vitamin B12 ELISA Kit
Vitamin B12 ELISA Kit for ELISA and Other
Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit
Overview
| Product Description | ARG80816 Vitamin B12 ELISA Kit is a Enzyme Immunoassay kit for the quantification of Vitamin B12 in food (extraction, dilution). |
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| Tested Reactivity | Other |
| Tested Application | ELISA |
| Target Name | Vitamin B12 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.3 ng/ml |
| Sample Type | Food (extraction, dilution). |
| Standard Range | 0.4 - 40 ng/ml |
| Sample Volume | 50 µl |
Application Instructions
| Assay Time | 1 h (RT/shaker), 20 min (RT/dark) |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Vitamin B12 |
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| Background | Vitamin B12 as a trace element belongs to the biologically important chelate formers. The basic unit consists of a corrin ring with cobalt as a central atom. Cobalt is sixfold coordinated by four nitrogen atoms, one cyanide and a dimethylbenzimidazol group. Vitamin B12 forms a stable complex, which is absorbed in the lower part of the small intestine, with the so-called intrinsic factor present in the gastric juice. A lack of vitamin B12 can lead among other things to pernicious anemia. This disease is not generated by an insufficient supply of vitamin B12, but by the absence of intrinsic factor. A pernicious anemia can be treated by a high dosage of vitamin B12. The existing detection procedures are mainly microbiological methods, but also HPLC and thin-layer chromatography, all of which are associated with a high amount of time and instrumentation. With the present test kit it is possible, to determine vitamin B12 quantitatively in vitaminated food in a significantly faster way (2.5 to 4 hours inclusive sample pretreatment) compared with a conventional microbiological assay (24 to 48 hours). |
| Highlight | Related products: Vitamin B12 antibodies; Vitamin B12 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
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ARG80816 Vitamin B12 ELISA Kit standard curve image
ARG80816 Vitamin B12 ELISA Kit results of a typical standard run with optical density reading at 450nm.
| Title | Download Link |
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| ARG80816 Vitamin B12 ELISA Kit User's manual |
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