ARG81246

VIP ELISA Kit

VIP ELISA Kit for ELISA and Human,Mouse,Rat,Guinea pig,Pig,Sheep

Overview

Product Description ARG81246 VIP ELISA Kit is an Enzyme Immunoassay kit for the quantification of VIP in serum and plasma.
Tested Reactivity Hu, Ms, Rat, Gpig, Pig, Sheep
Tested Application ELISA
Specificity Cross Reactivity: VIP (Human, Rat, Mouse, Porcine, Ovine and Guinea pig): 100%. VIP (10-28) (Human, Porcine, Rat): 34.6%. VIP (Chicken): 28%.
Target Name VIP
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.09 ng/ml
Detection Range Linear range: 0.09 - 0.81 ng/ml
Sample Type Serum and Plasma.
Standard Range 0.04 - 25 ng/ml
Sample Volume 50 µl
Precision Intra-Assay CV: less than 10%
Inter-Assay CV: less than 15%
Alternate Names Vasoactive intestinal polypeptide; PHM27; VIP peptides; Peptide histidine valine 42; VIP; Peptide histidine methioninamide 27

Application Instructions

Assay Time ~4.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117064 Rat VIP

GeneID: 22353 Mouse VIP

GeneID: 7432 Human VIP

Gene Symbol VIP
Gene Full Name vasoactive intestinal peptide
Background The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014]
Function VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.

PHM and PHV also cause vasodilation. PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin. [UniProt]
Highlight Related products:
VIP antibodies; VIP ELISA Kits; VIP Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG81246 VIP ELISA Kit standard curve image

    ARG81246 VIP ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81246 VIP ELISA Kit User's manual download icon Download