ARG83430
Urate Oxidase Assay Kit
Urate Oxidase Assay Kit for Functional study and Other
Overview
| Product Description | ARG83430 Urate Oxidase Assay Kit can be used to measure Urate oxidase in tissue extracts, cell lysate, cell culture media and other biological fluids. |
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| Tested Reactivity | Other |
| Tested Application | FuncSt |
| Target Name | Urate oxidase |
| Conjugation Note | Read at 550 nm |
| Sensitivity | 0.01 μmol/ml |
| Sample Type | Tissue extracts, cell lysate, cell culture media and other biological fluids. |
| Standard Range | 0.03 μmol/ml - 3 μmol/ml |
| Sample Volume | 10 μl |
| Alternate Names | factor-independent urate hydroxylase; uric acid oxidase; uricase; uricase II; urate oxidase |
Application Instructions
| Assay Time | 15 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C, -20°C, -80°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | This enzyme was previously thought to be a copper protein, but it is now known that the enzymes from soy bean (Glycine max), the mould Aspergillus flavus and Bacillus subtilis contains no copper nor any other transition-metal ion. The 5-hydroxyisourate formed decomposes spontaneously to form allantoin and CO2, although there is an enzyme-catalysed pathway in which EC 3.5.2.17, hydroxyisourate hydrolase, catalyses the first step. The enzyme is different from EC 1.14.13.113 (FAD-dependent urate hydroxylase). |
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| Title | Download Link |
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| ARG83430 Urate Oxidase Assay Kit User manual |
 Download
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