ARG23857

Swine anti-Goat IgG (H+L) antibody (HRP), pre-adsorbed

Swine anti-Goat IgG (H+L) antibody (HRP), pre-adsorbed for ELISA,Flow cytometry,FLISA,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Goat

publication_link Publication1

Overview

Product Description HRP-conjugated Pig Polyclonal antibody recognizes Goat IgG (H+L)
Tested Reactivity Goat
Tested Application ELISA, FACS, FLISA, IHC-Fr, IHC-P, WB
Specificity Reacts with the heavy and light chains of goat IgG
Host Pig
Clonality Polyclonal
Isotype IgG
Target Name IgG (H+L)
Antigen Species Goat
Conjugation HRP

Application Instructions

Pre Adsorbed Human, Rat, and Mouse serum proteins.
Application Suggestion
Tested Application Dilution
ELISA1:4000 - 1:8000
FACSAssay-dependent
FLISAAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4) and 50% Glycerol.
Stabilizer 50% Glycerol
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (4) Click the Picture to Zoom In

  • ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG21968 anti-Collagen IV antibody (pre-adsorbed) (right), followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.

  • ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted ARG21652 Goat anti-Human IgM antibody, F(ab')2 fragment (pre-adsorbed) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed).

  • ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG23772 Goat IgG Isotype Control antibody (left) and ARG20786 anti-Collagen III antibody (pre-adsorbed) (right) followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed), DAB and hematoxylin.

  • ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat anti-Human IgG antibody followed by ARG23857 Swine anti-Goat IgG (H+L) antibody (HRP) (pre-adsorbed).

Specific References

Contribution of neuronal calcium sensor 1 (Ncs-1) to anxiolytic-like and social behavior mediated by valproate and Gsk3 inhibition.

WB / Goat

Magno Luiz Alexandre Viana et al.
Sci Rep.,  (2020)

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