ARG82627
Soluble Collagen Assay Kit
Soluble Collagen Assay Kit for Functional study and All species
Overview
| Product Description | ARG82627 Soluble Collagen Assay Kit is a detection kit for the quantification of soluble or solubilized collagens in tissue culture supernatants, cellular extracts and tissue homogenates. |
|---|---|
| Tested Reactivity | All |
| Tested Application | FuncSt |
| Specificity | Measures all types of fibrillar collagen, species independent. |
| Target Name | Collagen |
| Conjugation Note | Read at 540 nm. |
| Detection Range | 0.5 - 10 µg/well (3.5 - 70 µg/ml) |
| Sample Type | Tissue culture supernatants, cellular extracts and tissue homogenates. |
| Sample Volume | 140 µl |
| Alternate Names | OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen |
Application Instructions
| Assay Time | < 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | COL1A1 |
|---|---|
| Gene Full Name | collagen, type I, alpha 1 |
| Background | This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| Highlight | Related products: Collagen antibodies; Collagen ELISA Kits; Collagen Duos / Panels; Collagen recombinant proteins; Related news: New antibody panels for Myofibroblasts and CAFs |
| PTM | Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82627 Soluble Collagen Assay Kit typical data demonstration image
ARG82627 Soluble Collagen Assay Kit results of a typical data with optical density reading at 540 nm.
| Title | Download Link |
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| ARG82627 Soluble Collagen Assay Kit User manual |
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