ARG21638

Rat anti-Mouse Kappa Light Chain antibody [H139-52.1] (Biotin)

Rat anti-Mouse Kappa Light Chain antibody [H139-52.1] (Biotin) for ELISA,Flow cytometry,FLISA,ICC/IF,Western blot and Mouse

Overview

Product Description Biotin-conjugated Rat Monoclonal antibody [H139-52.1] recognizes Mouse Kappa Light Chain
Tested Reactivity Ms
Tested Application ELISA, FACS, FLISA, ICC/IF, WB
Host Rat
Clonality Monoclonal
Clone H139-52.1
Isotype IgG1, kappa
Target Name Kappa Light Chain
Antigen Species Mouse
Conjugation Biotin

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21638 Rat anti-Mouse Kappa Light Chain antibody [H139-52.1] (Biotin) ELISA image

    ELISA: The plate was coated with purified Mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted ARG21638 Rat anti-Mouse Kappa Light Chain antibody [H139-52.1] (Biotin) followed by ARG23912 Streptavidin (HRP).

  • ARG21638 Rat anti-Mouse Kappa Light Chain antibody [H139-52.1] (Biotin) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21638 Rat anti-Mouse Kappa Light Chain antibody [H139-52.1] (Biotin) and ARG20852 anti-CD19 antibody [6D5] (PE) followed by Streptavidin (FITC).