ARG82544

Rat Semaphorin 6C ELISA Kit

Rat Semaphorin 6C ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82544-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82544-002 Standard 2 X 10 ng/vial 4°C
ARG82544-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82544-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82544-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82544-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82544-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82544-008 25X Wash buffer 20 ml 4°C
ARG82544-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82544-010 STOP solution 10 ml (Ready to use) 4°C
ARG82544-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82544 Rat Semaphorin 6C ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Semaphorin 6C in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Target Name Semaphorin 6C
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 50 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%
Inter-Assay CV: 6.8%
Alternate Names SEMAY; Semaphorin-6C; m-SemaY2; Semaphorin-Y; m-SemaY; Sema Y

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29744 Rat SEMA6C

Swiss-port # Q9WTL3 Rat Semaphorin-6C

Gene Symbol SEMA6C
Gene Full Name sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C
Background This gene encodes a member of the semaphorin family. Semaphorins represent important molecular signals controlling multiple aspects of the cellular response that follows CNS injury, and thus may play an important role in neural regeneration. [provided by RefSeq, May 2010]
Function Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Highlight Related products:
Semaphorin antibodies; Semaphorin ELISA Kits;
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Images (1) Click the Picture to Zoom In

  • ARG82544 Rat Semaphorin 6C ELISA Kit standard curve image

    ARG82544 Rat Semaphorin 6C ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82544 Rat Semaphorin 6C ELISA Kit User manual download icon Download