ARG81962
Rat Sclerostin ELISA Kit
Rat Sclerostin ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81962-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81962-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81962-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81962-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81962-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81962-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81962-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81962-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81962-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81962-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81962-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81962 Rat Sclerostin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Sclerostin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Sclerostin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3%; Inter-Assay CV: 5.7% |
| Alternate Names | CDD; SOST1; Sclerostin; VBCH |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SOST |
| Gene Full Name | sclerostin |
| Background | Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease. [provided by RefSeq, Jul 2008] |
| Function | Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. [UniProt] |
| Highlight | Related products: Sclerostin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81962 Rat Sclerostin ELISA Kit standard curve image
ARG81962 Rat Sclerostin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81962 Rat Sclerostin ELISA Kit User's manual |
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