ARG82397
Rat REG3A ELISA Kit
Rat REG3A ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82397-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82397-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82397-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82397-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82397-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82397-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82397-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82397-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82397-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82397-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82397-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82397 Rat REG3A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat REG3A in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | REG3A |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.8% Inter-Assay CV: 6.8% |
| Alternate Names | HIP; PAP; HIP/PAP; Regenerating islet-derived protein 3-alpha; PAP-H; REG-III; Hepatointestinal pancreatic protein; PBCGF; Human proislet peptide; REG3; INGAP; Regenerating islet-derived protein III-alpha; REG-3-alpha; Reg III-alpha; PAP1; Pancreatitis-associated protein 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P35231 Rat Regenerating islet-derived protein 3-alpha |
|---|---|
| Gene Symbol | REG3A |
| Gene Full Name | regenerating islet-derived 3 alpha |
| Background | This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Nov 2014] |
| Function | Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway. [UniProt] |
| Cellular Localization | Secreted. Note=Found in the apical region of pancreatic acinar cells. [UniProt] |
| Highlight | Related products: REG3A antibodies; REG3A ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82397 Rat REG3A ELISA Kit standard curve image
ARG82397 Rat REG3A ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82397 Rat REG3A ELISA Kit User manual |
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