Overview

Product Description	ARG83644 Rat RANTES ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat RANTES in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity	Rat
Tested Application	ELISA
Target Name	RANTES
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm.
Sensitivity	5 pg/ml
Sample Type	Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range	15.6 - 1000 pg/ml
Sample Volume	100 μl
Precision	Intra-Assay CV: < 10% Inter-Assay CV: < 10%
Alternate Names	CCL5; C-C Motif Chemokine Ligand 5; TCP228; SIS-Delta; D17S136E; RANTES; SCYA5; SISd; Regulated Upon Activation, Normally T-Expressed, And Presumably Secreted; Small Inducible Cytokine Subfamily A (Cys-Cys), Member 5; Eosinophil Chemotactic Cytokine; Chemokine (C-C Motif) Ligand 5; T-Cell Specific Protein P288; Beta-Chemokine RANTES; C-C Motif Chemokine 5; MGC17164; EoCP; Small Inducible Cytokine A5 (RANTES); T-Cell Specific RANTES Protein; T-Cell-Specific Protein RANTES; T Cell-Specific Protein P228; Small-Inducible Cytokine A5

Application Instructions

Assay Time	4 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 81780 Rat CCL5
Gene Symbol	CCL5
Gene Full Name	C-C Motif Chemokine Ligand 5
Background	This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
Function	May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells. [Uniprot]
Cellular Localization	Secreted. [Uniprot]
PTM	Disulfide bond, Glycoprotein, Oxidation. [Uniprot]

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