ARG81791

Rat RAGE ELISA Kit

Rat RAGE ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81791-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81791-002 Standard 2 X 10 ng/vial 4°C
ARG81791-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81791-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81791-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81791-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81791-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81791-008 25X Wash buffer 20 ml 4°C
ARG81791-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81791-010 STOP solution 10 ml (Ready to use) 4°C
ARG81791-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81791 Rat RAGE ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat RAGE in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name RAGE
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 39 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 78 - 5000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%; Inter-Assay CV: 7.1%
Alternate Names Receptor for advanced glycosylation end products; Advanced glycosylation end product-specific receptor; RAGE

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol AGER
Gene Full Name advanced glycosylation end product-specific receptor
Background RAGE: Advanced glycosylation end product (AGE) receptor is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]
Function RAGE Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides. [UniProt]
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RAGE antibodies; RAGE ELISA Kits;
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Images (1) Click the Picture to Zoom In

  • ARG81791 Rat RAGE ELISA Kit standard curve image

    ARG81791 Rat RAGE ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81791 Rat RAGE ELISA Kit User's manual download icon Download