ARG82572
Rat R Cadherin / Cadherin 4 ELISA Kit
Rat R Cadherin / Cadherin 4 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82572-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82572-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82572-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82572-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82572-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82572-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82572-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82572-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82572-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82572-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82572-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82572 Rat R Cadherin / Cadherin 4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat R Cadherin / Cadherin 4 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | R Cadherin / Cadherin 4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.2% Inter-Assay CV: 7.1% |
| Alternate Names | R-CAD; Cadherin-4; Retinal cadherin; CAD4; RCAD; R-cadherin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CDH4 |
|---|---|
| Gene Full Name | cadherin 4, type 1, R-cadherin (retinal) |
| Background | This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: Cadherin antibodies; Cadherin ELISA Kits; Cadherin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82572 Rat R Cadherin / Cadherin 4 ELISA Kit standard curve image
ARG82572 Rat R Cadherin / Cadherin 4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82572 Rat Cadherin 4 ELISA Kit User manual |
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