ARG82518

Rat PDGF CC ELISA Kit

Rat PDGF CC ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82518-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82518-002 Standard 2 X 10 ng/vial 4°C
ARG82518-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82518-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82518-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82518-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82518-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82518-008 25X Wash buffer 20 ml 4°C
ARG82518-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82518-010 STOP solution 10 ml (Ready to use) 4°C
ARG82518-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82518 Rat PDGF CC ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat PDGF CC in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Target Name PDGF CC
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 10 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.6%
Inter-Assay CV: 6.0%
Alternate Names Spinal cord-derived growth factor; Fallotein; PDGFC receptor-binding form; SCDGF; FALLOTEIN; VEGF-E; Platelet-derived growth factor C; PDGFC latent form; PDGF-C

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 79429 Rat PDGFC

Swiss-port # Q9EQX6 Rat Platelet-derived growth factor C

Gene Symbol PDGFC
Gene Full Name platelet derived growth factor C
Background The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines. This gene product appears to form only homodimers. It differs from the platelet-derived growth factor alpha and beta polypeptides in having an unusual N-terminal domain, the CUB domain. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2010]
Function Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. [UniProt]
Cellular Localization Cytoplasm, cytosol. Secreted. Nucleus. Cytoplasmic granule. Cell membrane. Note=Sumoylated form is predominant in the nucleus (PubMed:15247255). Stored in alpha granules in platelets (PubMed:15061151). [UniProt]
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PTM Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after basic residues in the hinge region (region connecting the CUB and growth factor domains) gives rise to the receptor-binding form. Cleaved by PLAT and PLG.

Sumoylated with SUMO1.

N-glycosylated. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82518 Rat PDGF CC ELISA Kit standard curve image

    ARG82518 Rat PDGF CC ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82518 Rat PDGF CC ELISA Kit User manual download icon Download